pet28a-mbp vectors Search Results


pet28a  (Addgene inc)
94
Addgene inc pet28a
Pet28a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet28a-mbp+vectors/pET+His6+MBP+TEV+LIC+cloning+vector+(1M)+(Plasmid+%2329656)/pm28516954-157-35-48
Average 94 stars, based on 1 article reviews
pet28a - by Bioz Stars, 2026-09
94/100 stars
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pet28a-mbp vectors  (GenScript corporation)
90
GenScript corporation pet28a-mbp vectors
Pet28a Mbp Vectors, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet28a-mbp+vectors/pet28a/pm35417705-278-29-31
Average 90 stars, based on 1 article reviews
pet28a-mbp vectors - by Bioz Stars, 2026-09
90/100 stars
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pet28a mbp tev vectors  (Addgene inc)
92
Addgene inc pet28a mbp tev vectors
E. coli -produced recombinant Can f 6 variant expression and purification. a, b Schematic illustrations of gene expression constructs. a <t>pET28a(</t> +)-Can f 6 expression vector features the Can f 6 gene tagged at the N-terminus with a 6xHis tag. b pET28a( +)-MBP-TEV-Can f 6 expression vector features Can f 6 fused to the C-terminus of a 6xHis-MBP fusion protein, with a TEV protease cleavage site inserted between the fusion partner and the target protein. SDS-PAGE analysis of expression and purification of c rCan f 6 and d rMBP-Can f 6. Lanes: (1), lysate fraction from non-induced E. coli ; (2), lysate fraction from IPTG-induced E. coli ; (3), soluble fraction before purification with nickel-chelate chromatography; (4), soluble fraction after incubation with Ni–NTA agarose; (5–9), wash fractions; (10–14), elution fractions containing purified rCan f 6 and rMBP-Can f 6 proteins, respectively. M—Pierce™ Unstained Protein MW Marker (Thermo Fisher Scientific, USA). Proteins were separated on SDS-PAGE gels of c 12% and d 14% acrylamide concentrations
Pet28a Mbp Tev Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet28a-mbp+vectors/pET28a(%2B)+6xHis-TEV-IFIT1+(Plasmid+%2353557)/pmc11954706-50-20-30
Average 92 stars, based on 1 article reviews
pet28a mbp tev vectors - by Bioz Stars, 2026-09
92/100 stars
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pet28a mbp tev vector  (Addgene inc)
93
Addgene inc pet28a mbp tev vector
E. coli -produced recombinant Can f 6 variant expression and purification. a, b Schematic illustrations of gene expression constructs. a <t>pET28a(</t> +)-Can f 6 expression vector features the Can f 6 gene tagged at the N-terminus with a 6xHis tag. b pET28a( +)-MBP-TEV-Can f 6 expression vector features Can f 6 fused to the C-terminus of a 6xHis-MBP fusion protein, with a TEV protease cleavage site inserted between the fusion partner and the target protein. SDS-PAGE analysis of expression and purification of c rCan f 6 and d rMBP-Can f 6. Lanes: (1), lysate fraction from non-induced E. coli ; (2), lysate fraction from IPTG-induced E. coli ; (3), soluble fraction before purification with nickel-chelate chromatography; (4), soluble fraction after incubation with Ni–NTA agarose; (5–9), wash fractions; (10–14), elution fractions containing purified rCan f 6 and rMBP-Can f 6 proteins, respectively. M—Pierce™ Unstained Protein MW Marker (Thermo Fisher Scientific, USA). Proteins were separated on SDS-PAGE gels of c 12% and d 14% acrylamide concentrations
Pet28a Mbp Tev Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet28a-mbp+vectors/pET28a(%2B)%3A%3AMBP-NL3F10H+(Plasmid+%23141291)/10__1016_slash_j__chempr__2025__102438-198-1-3
Average 93 stars, based on 1 article reviews
pet28a mbp tev vector - by Bioz Stars, 2026-09
93/100 stars
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Image Search Results


E. coli -produced recombinant Can f 6 variant expression and purification. a, b Schematic illustrations of gene expression constructs. a pET28a( +)-Can f 6 expression vector features the Can f 6 gene tagged at the N-terminus with a 6xHis tag. b pET28a( +)-MBP-TEV-Can f 6 expression vector features Can f 6 fused to the C-terminus of a 6xHis-MBP fusion protein, with a TEV protease cleavage site inserted between the fusion partner and the target protein. SDS-PAGE analysis of expression and purification of c rCan f 6 and d rMBP-Can f 6. Lanes: (1), lysate fraction from non-induced E. coli ; (2), lysate fraction from IPTG-induced E. coli ; (3), soluble fraction before purification with nickel-chelate chromatography; (4), soluble fraction after incubation with Ni–NTA agarose; (5–9), wash fractions; (10–14), elution fractions containing purified rCan f 6 and rMBP-Can f 6 proteins, respectively. M—Pierce™ Unstained Protein MW Marker (Thermo Fisher Scientific, USA). Proteins were separated on SDS-PAGE gels of c 12% and d 14% acrylamide concentrations

Journal: Applied Microbiology and Biotechnology

Article Title: Enhancing production and assessing IgE reactivity of dog allergen Can f 6 in Pichia pastoris and Escherichia coli

doi: 10.1007/s00253-025-13465-7

Figure Lengend Snippet: E. coli -produced recombinant Can f 6 variant expression and purification. a, b Schematic illustrations of gene expression constructs. a pET28a( +)-Can f 6 expression vector features the Can f 6 gene tagged at the N-terminus with a 6xHis tag. b pET28a( +)-MBP-TEV-Can f 6 expression vector features Can f 6 fused to the C-terminus of a 6xHis-MBP fusion protein, with a TEV protease cleavage site inserted between the fusion partner and the target protein. SDS-PAGE analysis of expression and purification of c rCan f 6 and d rMBP-Can f 6. Lanes: (1), lysate fraction from non-induced E. coli ; (2), lysate fraction from IPTG-induced E. coli ; (3), soluble fraction before purification with nickel-chelate chromatography; (4), soluble fraction after incubation with Ni–NTA agarose; (5–9), wash fractions; (10–14), elution fractions containing purified rCan f 6 and rMBP-Can f 6 proteins, respectively. M—Pierce™ Unstained Protein MW Marker (Thermo Fisher Scientific, USA). Proteins were separated on SDS-PAGE gels of c 12% and d 14% acrylamide concentrations

Article Snippet: Subsequent cloning of the resultant DNA fragment occurred at the BamH I and Xho I sites within pET28a( +) and pET28a( +)-MBP-TEV vectors, gifts from Zita Balklava & Thomas Wassmer (Addgene plasmid #69,929; http://n2t.net/addgene:69929 ; RRID:Addgene_69929) (Currinn et al. ).

Techniques: Produced, Recombinant, Variant Assay, Expressing, Purification, Gene Expression, Construct, Plasmid Preparation, SDS Page, Chromatography, Incubation, Marker